Laboratory of Molecular Genetics
Director: Dr. Sotiria Boukouvala, Associate Professor
Building 10, University Campus,
Tel.: +30-25510-30632, Fax. (Departmental): +30-25510-30613, email: firstname.lastname@example.org
The group undertakes comparative genomic and functional investigations of enzymes that modulate the effects of chemicals (drugs, carcinogens, pollutants, toxic agents etc.) on living organisms. Current projects investigate a family of xenobiotic metabolizing enzymes, called arylamine N-acetyltransferases (NATs), which serve diverse metabolic functions in prokaryotic and eukaryotic microorganisms of medical and/or biotechnological relevance. In humans, the highly polymorphic NAT genes have been landmark examples of how genetic variability may determine individual susceptibility to chemical carcinogenesis and drug induced toxicity.
The group also
maintains a strong biomedical-biotechnological orientation, building on the
director's previous experience in the diagnostics and the pharmaceutical
industry. Research ventures in that area have included cross-border collaboration with
Expertise and infrastructure
The laboratory shares its infrastructure with the Laboratory of Human Genetics & Model Organisms and is fully equipped to support methodologies ranging from in silico genomic surveys to advanced molecular genetic and biochemical applications. Current expertise includes high-throughput PCR and real-time PCR, cloning, protein expression and purification, western blot, various spectrophotometric, chemiluminescent and fluorescent assays, differential scanning fluorimetry, pulse field gel electrophoresis, cell culture, microbiology, etc.
Available equipment includes a DNA 4x96 Engine Tetrad for PCR (MJ), a TECAN M1000 reader for colorimetric, chemiluminescent or fluorescent in vitro/cell-based assays, a BioRad ChemiDoc XRS+ Imaging System for gels and chemiluminsescent blots, a Nanodrop spectrophotometer (Thermo), a BioRad Experion Automated System for chip-based electrophoresis and qualitative/quantitative analysis of RNA/DNA and protein, various specialized electrophoresis instruments (e.g. for PFGE, E-gels etc.), electroporator, sonicator, various incubators, -80°C freezer, etc.
The laboratory has three dedicated areas, one for PCR (with sterile hood), a second for microbiology (equipped with a BL2 hood, incubator and microscopes) and a third for cell culture (equipped with a BL2-Cytotoxic hood, inverted microscope, CO2 incubator and accessory instruments). Access to real-time PCR equipment and communal state-of-the-art departmental facilities (e.g. surface plasmon resonance technology and confocal microscopy) is also available locally.
Current International Collaborations
· Prof. (emer.) Edith
Sim (Department of Pharmacology,
· Prof. Károly
Márialigeti (Department of Microbiology, Faculty of Biology,
· Dr. Anthony E. Glenn (Toxicology & Mycotoxin Research Unit, Agricultural Research Service, US Department of Agriculture, Athens, GA, USA)
Dr. Audrey Sabbagh (Université
· Dr. Estella Poloni (Department of Genetics and Evolution, Université de Genève)
Prof. Rodney F. Minchin (School of Biomedical Sciences,
David W. Hein (Department of Pharmacology & Toxicology,